Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8300268 | Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms | 2018 | 25 Pages |
Abstract
Ribosome-associated noncoding (ranc) RNAs are a novel class of short regulatory RNAs with functions and origins that have not been well studied. In this present study, we functionally characterized the molecular activity of Saccharomyces cerevisiae transfer RNA (tRNA)-derived fragments (tRFs) during protein biosynthesis. Our results indicate ribosome-associated tRFs derived from both 5â² (ranc-5â²-tRFs) and 3â²-part of tRNAs (ranc-3â²-tRFs) have regulatory roles during translation. We demonstrated five 3â²-tRFs and one 5â²-tRF associate with a small ribosomal subunit and aminoacyl-tRNA synthetases (aa-RSs) in yeast. Furthermore, we discovered that four yeast aa-RSs interact directly with yeast ribosomes. tRFs interactions with ribosome-associated aa-RSs correlate with impaired efficiency of tRNA aminoacylation.
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Authors
Anna M. Mleczko, Piotr Celichowski, Kamilla BÄ
kowska-Żywicka,