| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8300899 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2018 | 31 Pages |
Abstract
The study identified a loop of GH5 for mannan recognition and catalysis. Weakening the hydrophobic environment near the loop can also enhance the enzyme catalytic rate. Our findings provide a new insight on mannan recognition and activity enhancement of GH5.
Keywords
BeechwoodHEPESDTTPDBOD600TLCCMCTRI4-(2-hydroxyethyl)-1-piperazineethanesulfonic acidX-ray crystallographyoptical density at 600 nmTris(hydroxymethyl)aminomethaneSubstrate specificitydithiothreitolBiofuelLuria-BertaniProtein engineeringProtein Data BankCarboxymethyl cellulosethin-layer chromatographyglycoside hydrolase
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Authors
Po-Huang Liang, Wen-Ling Lin, Han-Yu Hsieh, Tsung-Yi Lin, Chun-Hsu Chen, Sunil K. Tewary, Hsiao-Lin Lee, Shuo-Fu Yuan, Barbara Yang, Jyun-Yu Yao, Meng-Chiao Ho,