| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8301200 | Biochimica et Biophysica Acta (BBA) - General Subjects | 2019 | 13 Pages |
Abstract
⺠Changes in glycogene expression were found during NT2N neuron differentiation. ⺠Increase in FUT9 expression that encodes the enzyme synthesizing Lewisx was observed. ⺠NCAM is the predominant glycoprotein carrier of Lewisx in NT2N neurons. ⺠Downregulation of FUT9 caused decreased Lewisx levels and impaired neurite outgrowth.
Keywords
PBSdHexHexNAcLeXHNK-1PNGase FHPAEC-PADHRPTBSEGTAGlcNAcFucoseFucBSAN-acetylhexosaminebovine serum albuminEDTAethylene glycol tetraacetic acidSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisTris-buffered salineNeuronal differentiationdeoxyhexoseNeurite outgrowthLewisxMALDI-TOF MSMannosePhosphate-buffered salineManN-acetylglucosamineHexHexoseHorseradish peroxidasepeptide N-glycosidase FGalGalactose
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Authors
Ricardo Gouveia, Lana Schaffer, Suzanne Papp, Nicolas Grammel, Sebastian Kandzia, Steven R. Head, Ralf Kleene, Melitta Schachner, Harald S. Conradt, Júlia Costa,