Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8301277 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2018 | 41 Pages |
Abstract
The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilities of a novel GPR119 agonist, Oleoyl-lysophosphatidylinositol (Oleoyl-LPI). Oleoyl-LPI is the only LPI species able to potently stimulate the release of GLP-1 in vitro, from murine and human L-cells, and ex-vivo from murine colonic primary cell preparations. Here we show that Oleoyl-LPI mediates GLP-1 secretion through GPR119 as this activity is ablated in cells lacking GPR119 and in colonic primary cell preparation from GPR119â/â mice. Similarly, Oleoyl-LPI-mediated insulin secretion is impaired in islets isolated from GPR119â/â mice. On the other hand, GLP-1 secretion is not impaired in cells lacking GPR55 in vitro or in colonic primary cell preparation from GPR55â/â mice. We therefore conclude that GPR119 is the Oleoyl-LPI receptor, upstream of ERK1/2 and cAMP/PKA/CREB pathways, where primarily ERK1/2 is required for GLP-1 secretion, while CREB activation appears dispensable.
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Authors
Syamsul A. Arifin, Silvano Paternoster, Rodrigo Carlessi, Ilaria Casari, Jeppe Hvidtfeldt Ekberg, Tania Maffucci, Philip Newsholme, Mette M. Rosenkilde, Marco Falasca,