| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8301828 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2015 | 29 Pages |
Abstract
Dolichols are, among others, obligatory cofactors of protein glycosylation in eukaryotic cells. It is well known that yeast cells accumulate a family of dolichols with Dol-15/16 dominating while upon certain physiological conditions a second family with Dol-21 dominating is noted. In this report we identified the presence of additional short-chain length polyprenols - all-trans Pren-7 in three yeast strains (SS328, BY4741 and L5366), Pren-7 was accompanied by traces of putative Pren-6 and -8. Moreover, in two of these strains a single polyprenol mainly-cis-Pren-11 was synthesized at the stationary phase of growth. Identity of polyprenols was confirmed by HR-HPLC/MS, NMR and metabolic labeling. Additionally, simvastatin inhibited their biosynthesis.
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Authors
Liliana Surmacz, Jacek Wojcik, Magdalena Kania, Magnus Bentinger, Witold Danikiewicz, Gustav Dallner, Przemyslaw Surowiecki, Piotr Cmoch, Ewa Swiezewska,