| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8301877 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2015 | 10 Pages |
Abstract
Addition of free fatty acids to differentiation medium increased their incorporation during the first period of differentiation (48Â h). Presence of eicosanoid acid (EPA) during the initial period of differentiation by elevation of Perilipin 3 protein (TIP47), may be responsible for smaller LD formation. Presence of arachidonic acid (AA) tends to deposit lipids in large form of LDs. Prolongation of differentiation up to 15Â days decreased AA or EPA in cellular lipids. PUFA through up-regulation of both phospholipase 2 and enzymes related to eicosanoid production influenced type and quantity of eicosanoids which regulated the extent of SVF cell differentiation. Formation of small LDs and reduction of pro-inflammatory mediators in adipose tissue are the consequence of eicosanoid production with anti-inflammatory potential from EPA.
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Authors
Anna Polus, Beata Kiec-Wilk, Urszula Razny, Anna Gielicz, Gerd Schmitz, Aldona Dembinska-Kiec,