Characterization of a mutation that results in independence of oxidosqualene cyclase (Erg7) activity from the downstream 3-ketoreductase (Erg27) in the yeast ergosterol biosynthetic pathway

► We identify a novel mutation allowing independence of Erg7 from Erg27. ► We sequenced the ERG27 mutation and found a non synonymous alteration at position 598. ► We constructed a fusion plasmid that allowed retention of Erg7 in the ER.