| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8303067 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2012 | 10 Pages |
Abstract
⺠First crystal structure of monoacylglycerol lipase from bacterial species. ⺠Small angle X-ray scattering shows that the protein is a monomer in solution. ⺠A large hydrophobic channel enables access of the substrate to the active site. ⺠Molecular dynamic simulations reveal open and closed states of the cap region. ⺠The cap architecture is conserved on a structural but not on a sequence level.
Keywords
RMSDPMSFmGlOpen conformationSAXSSDS-PAGEsodium dodecyl sulphate polyacrylamide gel electrophoresisX-ray crystallographyEvolutionary conservationMolecular dynamics simulationphenylmethylsulfonyl fluoridemonoacylglycerol lipaseroot mean square deviationSmall-angle X-ray scatteringsmall angle X-ray Scattering
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Authors
Srinivasan Rengachari, Gustavo A. Bezerra, Lina Riegler-Berket, Christian C. Gruber, Christian Sturm, Ulrike Taschler, Andras Boeszoermenyi, Ingrid Dreveny, Robert Zimmermann, Karl Gruber, Monika Oberer,