NGF blocks polyunsaturated fatty acids biosynthesis in n − 3 fatty acid-supplemented PC12 cells

► PC12 cells are deficient in n−3 DHA due to lack of appropriate precursors. ► α-linolenic acid supplements overcome deficiency particularly in aminophospholipids. ► Linoleic acid does not affect α-linolenic acid intermediates biosynthesis. ► Nerve growth factor impairs denovo synthesis of n−3 and n−6 fatty acids metabolites.