Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8303584 | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | 2006 | 9 Pages |
Abstract
A 1.5-kb genomic DNA fragment corresponding to the 5â²-flanking region of the rat LDL receptor gene was cloned and putative regulatory regions were identified. A major transcription start site was identified at â154 bp relative to the ATG translation initiation codon, within a region containing two thyroid hormone response element half-site motifs (2H-TRE). Binding of thyroid hormone receptors α and β1 to this element was demonstrated. Mutations within this 2H-TRE region abolished basal transcription levels of the rat LDL receptor gene. Reporter gene studies indicated that the promoter region between â300 and â200 bp, which contains one sterol response element (SRE) and two specificity protein-1 sites (Sp1) sites, is crucial for basal transcription of the rat LDL receptor gene. The functionality of the SRE motif was confirmed using electrophoretic mobility shift assays and reporter gene studies.
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Authors
Dayami Lopez, Gene C. Ness,