Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8304144 | Biochimie | 2018 | 35 Pages |
Abstract
p-Cresyl glucuronide (p-CG) is a by-product of tyrosine metabolism that accumulates in patients with end-stage renal disease. p-CG binding to human serum albumin in physiological conditions (37â¯Â°C, pH 7.40) was studied by ultrafiltration (MWCO 10â¯kDa) and data were analyzed assuming one binding site. The estimated value of the association constant was 2.77â¯Ãâ¯103â¯Mâ1 and a maximal stoichiometry of 3.80â¯mol per mole. At a concentration relevant for end-stage renal patients, p-CG was 23% bound to albumin. Competition experiments, using fluorescent probes, demonstrated that p-CG did not bind to Sudlow's site I or site II. The p-CG did not interfere with the binding of p-cresyl-sulfate or indoxyl sulfate to serum albumin.
Keywords
PBSNSB3-carboxy-4-methyl-5-propyl-2-furanpropanoic acidCMPFHSAEGFRhuman serum albuminuremiachronic kidney diseaseESRD یا end stage renal diseaseEnd-stage renal diseaseuremic toxinPCsNon specific bindingPhosphate buffered salineestimated glomerular filtration rateCKDp-cresyl sulfateProtein bindingHigh pressure liquid chromatographyHPLC
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Authors
Dan Yi, Elisa Bernardes Monteiro, Stéphane Chambert, Hédi A. Soula, Julio B. Daleprane, Christophe O. Soulage,