Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8304222 | Biochimie | 2018 | 30 Pages |
Abstract
Browning of white adipocytes (beiging) is an attractive therapeutic strategy against obesity and its associated metabolic complications. Nobiletin (NOB) is a polymethoxylated flavone present in citrus fruits and has been reported to have anti-obesity effects. Here, we report that nobiletin exerts dual modulatory effects on adipocytes via induction of browning in 3T3-L1 white adipocytes and amelioration of stress in adipocytes. Nobiletin-induced beiging was investigated by determining expression levels of beige-specific genes and proteins by RT-PCR and immunoblot analysis, respectively. Nobiletin treatment rapidly elevated the expression levels of beige-specific genes such as Cd137, Cidea, Tbx1, and Tmem26. Further, nobiletin enhanced expression of the key transcription factors C/EBPβ, PPARδ, and PPARα, which are responsible for remodeling of white adipocytes. Nobiletin also strikingly activated HIB1B brown adipocytes and induced mitochondrial biogenesis in 3T3-L1 white adipocytes. In addition, nobiletin altered the expression of several lipid metabolism-related proteins such as ACOX1, CPT1, FAS, p-PLIN, SREBP and SIRT1. Moreover, nobiletin ameliorated stress in adipocytes by inhibiting expression levels of key stress molecules such as JNK and c-JUN. Nobiletin-induced browning could be mediated by tight regulation of kinases, as nobiletin induced PKA and p-AMPK at the protein expression level, and inhibition of PKA and p-AMPK by H-89 and dorsomorphin, respectively, abolished expression of the thermogenic markers PGC-1α and UCP1. Taken together, our findings suggest that nobiletin plays a modulatory role in adipocytes via induction of browning in 3T3-L1 white adipocytes and activation of HIB1B brown adipocytes combined with amelioration of stress in adipocytes, thereby exhibiting therapeutic potential against obesity.
Keywords
FGF21PR domain-containing 16/encoding genePRDM16/Prdm16autophagy proteinTMEM26Fat browningHSLAMPKPGC-1αATGACCCPTPPARACOpKaBAT3T3-L1 adipocytesAMP-activated protein kinaseacetyl-CoA carboxylaseWhite adipose tissuebrown adipose tissueAnti-obesityhormone-sensitive lipaseNobiletintransmembrane protein 26protein kinase AperilipinWATCarnitine palmitoyltransferasePLINCideaperoxisome proliferator-activated receptor
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Authors
Jameel Lone, Hilal Ahmad Parray, Jong Won Yun,