Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8305493 | Biochimie | 2014 | 4 Pages |
Abstract
IL-32 has been studied for its pleiotropic effects ranging from host immune responses to cell differentiation. Although several IL-32 isoforms have been characterized for their effects on cells, the roles of the others remain unclear. We previously reported that IL-32δ interacted with IL-32β and inhibited IL-32β-mediated IL-10 production. Thus, we performed comprehensive analyses to reveal more interactions between IL-32 isoforms in this study. We screened the interactions of 81 combinations of nine IL-32 isoforms by using a yeast two-hybrid assay, which identified 13 heterodimeric interactions. We verified these results by using reciprocal immunoprecipitation assays and reconfirmed 10 interactions, and presented the interaction network map between IL-32 isoforms. Our data suggest that IL-32 may have diverse intracellular effects through the interactions with its different isoforms.
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Authors
Jeong-Woo Kang, Yun Sun Park, Dong Hun Lee, Man Sub Kim, Yesol Bak, Sun Young Ham, Su Ho Park, Heejong Kim, Joong Hoon Ahn, Jin Tae Hong, Do-Young Yoon,