Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8306057 | Biochimie Open | 2017 | 8 Pages |
Abstract
We report the cloning and transient expression in HeLa cells of the five members of the human steroid 5α-reductase family as both N- and C-terminus green fluorescent protein tagged protein constructs. Following the intrinsic fluorescence of the tag, we have determined that the subcellular localization of these enzymes is in the endoplasmic reticulum, upon expression in HeLa cells. The presence of the tag at either end of the polypeptide chain can affect protein expression and, in the case of trans enoyl-CoA reductase, it induces the formation of protein aggregates.
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Authors
Antonella Scaglione, Linda Celeste Montemiglio, Giacomo Parisi, Italia Anna Asteriti, Renato Bruni, Gabriele Cerutti, Claudia Testi, Carmelinda Savino, Filippo Mancia, Patrizia Lavia, Beatrice Vallone,