Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8309515 | Clinica Chimica Acta | 2018 | 7 Pages |
Abstract
In this study, we developed a colorimetric method which is primarily based on the limited amount of redox in response to the miRNA target analyte binding for the determination of the miRNA, which is used as a biomarker to confirm the clinical diagnosis of cancers such as breast cancer. This method is on the basis of the peroxidase-like property of DNA templated copper nanoclusters (DNA-CuNCs). In this research, it was found that the DNA (poly T) stabilized green emitting CuNCs which exhibited enzyme-like peroxidase activity. After hybridization of miRNA with DNA-CuNCs probe and duplex formation, CuNCs catalyzed the oxidation of the methylene blue (MB) substrate, which can interact easily with DNA/miR-155 heteroduplex. Applying the optimal conditions, the absorbance of MB decreased by increasing the target miRNA-155 with in a dynamic range from 1.0â¯pM to 10.0â¯nM down to a detection limit (LOD) of 0.6â¯pM. The DNA-CuNCs/MB complex was designed to develop a facile, cheap, and fast colorimetric assay for detection of miRNA through MB oxidation by DNA stabilized CuNCs.
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Authors
Yasaman-Sadat Borghei, Morteza Hosseini, Mohammad Reza Ganjali,