| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8310786 | Clinica Chimica Acta | 2015 | 6 Pages |
Abstract
The present study presents external standardisation by spiking with non-human DNA fragments to control for loss of DNA during sample preparation and measurement. It also suggests a method to control for admixture of DNA from normal lymphocytes by utilizing the unique immunoglobulin gene rearrangement in the B-cells. The results show that this approach improves the quality of the analysis and lowers the risk of falsely increased values. In conclusion we suggest a new method to improve the accuracy of cfDNA measurements easily incorporated in the current technology.
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Authors
Niels Pallisgaard, Karen-Lise Garm Spindler, Rikke Fredslund Andersen, Ivan Brandslund, Anders Jakobsen,