Fast LC-MS/MS analysis of free oxysterols derived from reactive oxygen species in human plasma and carotid plaque

Article ID	Journal	Published Year	Pages	File Type
8312919	Clinica Chimica Acta	2013	26 Pages	PDF

Abstract

This rapid LC-MS/MS method enables reliable quantification focused on especially ROS-derived oxysterols in human plasma and atherosclerotic plaque samples under high-throughput conditions.

Keywords

LLE CXP Na2SO4 7-KC MQC SPE MRM APCI PQC BHT NaOH NQC LLOQ HCl CID LQC HQC GC-MS 7-Ketocholesterol CeA LC–MS/MS ROS S/N Solid phase extraction Liquid–liquid extraction hydrochloric acid Carotid endarterectomy collision energy Oxysterol collision induced dissociation LOD یا Limit of detection Triol Sodium sulfate Coefficient of Variation Gas Chromatography Mass Spectrometry Tandem mass spectrometry limit of detection Signal-to-noise ratio butylated hydroxytoluene sodium hydroxide declustering potential Atherosclerotic plaque Fast chromatography liquid chromatography liquid chromatography tandem mass spectrometry high-performance liquid chromatography HPLC Cholestane-3β,5α,6β-triol quality controls Reactive oxygen species atmospheric pressure chemical ionization

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

Fast LC-MS/MS analysis of free oxysterols derived from reactive oxygen species in human plasma and carotid plaque

Authors

Christin Helmschrodt, Susen Becker, Jenny Schröter, Max Hecht, Gabriela Aust, Joachim Thiery, Uta Ceglarek,