Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8313519 | Clinica Chimica Acta | 2013 | 7 Pages |
Abstract
BackgroundSalt sensitivity (SS) of blood pressure (BP) affects 25% of adults, shares comorbidity with hypertension, and has no convenient diagnostic test. We tested the hypothesis that urine-derived exfoliated renal proximal tubule cells (RPTCs) could diagnose the degree of an individual's SS of BP.MethodsSubjects were selected who had their SS of BP determined 5 y prior to this study (salt-sensitive: â¥Â 7 mm Hg increase in mean arterial pressure (MAP) following transition from a random weekly diet of low (10 mmol/day) to high (300 mmol/day) sodium (Na+) intake, N = 4; inverse salt-sensitive (ISS): â¥Â 7 mm Hg increase in MAP transitioning from a high to low Na+ diet, N = 3, and salt-resistant (SR): < 7 mm Hg change in MAP transitioned on either diet, N = 5). RPTC responses to 2 independent Na+ transport pathways were measured.ResultsThere was a negative correlation between the degree of SS and dopamine-1 receptor (D1R) plasma membrane recruitment (y = â 0.0107x + 0.68 relative fluorescent units (RFU), R2 = 0.88, N = 12, P < 0.0001) and angiotensin II-stimulated intracellular Ca++ (y = â 0.0016x + 0.0336, R2 = 0.7112, P < 0.001, N = 10) concentration over baseline.ConclusionsIsolating RPTCs from urine provides a personalized cell-based diagnostic test of SS index that offers advantages over a 2-week controlled diet with respect to cost and patient compliance. Furthermore, the linear relationship between the change in MAP and response to 2 Na+ regulatory pathways suggests that an individual's RPTC response to intracellular Na+ is personalized and predictive.
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Authors
John J. Gildea, Dylan T. Lahiff, Robert E. Van Sciver, Ryan S. Weiss, Neema Shah, Helen E. McGrath, Cynthia D. Schoeffel, Pedro A. Jose, Robert M. Carey, Robin A. Felder,