Diagnostic and clinical utility of antibodies against the nuclear body promyelocytic leukaemia and Sp100 antigens in patients with primary biliary cirrhosis

► Nuclear body sp100 autoantibodies are diagnostic markers of primary biliary cirrhosis (PBC). ► A robust assay for the testing of nuclear body PML autoantibodies is missing. ► A new line immunoassay simultaneously detecting PML and sp100 autoantibodies has been developed. ► PML/sp100 autoantibody positive PBCs have more advanced disease compared to seronegative. ► Multi-parametric testing of nuclear autoantibodies is a useful immunodiagnostic tool in PBC.