| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8314871 | Clinica Chimica Acta | 2012 | 5 Pages |
Abstract
⺠The analysis of 25-hydroxyvitamin D by LC-MS/MS is not straightforward. ⺠Greater variability was observed between laboratories using ESI compared to APCI. ⺠Using water-loss fragmentation increased variability between laboratories. ⺠Only 5% of laboratories could resolve interfering 3-epi-25-hydroxyvitamin D. ⺠Participation in an EQA scheme is essential for this assay.
Keywords
ESI4-Phenyl-1,2,4-triazoline-3,5-dionePTAD25-Hydroxyvitamin D225OHD3vitamin D External Quality Assessment SchemeDEQASpentafluorophenyl25OHDMRMPFPAPPIAPCISRM25-hydroxyvitamin D25-hydroxyvitamin D3LC–MS/MSm/zStandardisationCoefficient of VariationMass-to-charge ratioselected reaction monitoringmultiple reaction monitoringliquid chromatography–tandem mass spectrometryHPLChigh-performance liquid chromatographyelectrospray ionisationAtmospheric pressure chemical ionisation
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Authors
Lewis Couchman, Christopher M. Benton, Cajetan F. Moniz,