Simultaneous quantification of four vitamin D metabolites in human serum using high performance liquid chromatography tandem mass spectrometry for vitamin D profiling

Article ID	Journal	Published Year	Pages	File Type
8317838	Clinical Biochemistry	2012	6 Pages	PDF

Abstract

âº HPLC-MS/MS method for vitamin D profiling in human serum samples âº Simultaneous quantification of 25OH-D3, 3-epi-25OH-D3, 25OH-D2 and 24R,25(OH)2-D3 âº Chromatographic separation of 25OH-D3 and 3-epi-25OH-D3 below 10Â min âº Significant amounts of 3-epi-25OH-D3 and 24R,25(OH)2-D3 in serum samples detected. âº 24R,25(OH)2-D3 level increased disproportionally with increasing 25OH-D3 level.

Keywords

25-Hydroxyvitamin D2 25-hydroxyvitamin D3

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

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Simultaneous quantification of four vitamin D metabolites in human serum using high performance liquid chromatography tandem mass spectrometry for vitamin D profiling

Authors

Silvia Baecher, Andreas Leinenbach, Jo Anne Wright, Stephan Pongratz, Uwe Kobold, Roland Thiele,