Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8318764 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2018 | 40 Pages |
Abstract
Desaturase enzymes play an important role in the synthesis of unsaturated fatty acids. In this study, a complete cDNA sequence of a Î6 desaturase-like gene was cloned from the hepatopancreas of the red claw crayfish, Cherax quadricarinatus. The full-length 1885â¯bp sequence comprises a 5â² UTR of 254â¯bp, 3â² UTR of 234â¯bp, and an open reading frame (ORF) of 1377â¯bp encoding a 458 amino acid polypeptide (GenBank accession no. MF497442). Bioinformatics analysis revealed three conserved histidine-rich regions, a cytochrome b5 domain at the N-terminus, and a haem binding site (HPGG) in the cytochrome b5 domain, all of which are typical of Î6 desaturases. Quantitative real-time PCR demonstrated significantly higher expression in the hepatopancreas than other tissues. After feeding crayfish four formulated diets in which fish oil was replaced by 0, 33, 67, or 100% highly unsaturated soybean oil for 8â¯weeks, Î6 desaturase-like mRNA expression and enzyme activity were higher than in the fish oil only group. Additionally, a 4-week low temperature treatment at 25, 20, 15, or 9â¯Â°C increased Î6 desaturase mRNA expression and enzyme activity with decreasing water temperature. These results may help us better understand the biosynthesis of unsaturated fatty acids in C. quadricarinatus.
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Authors
Dong-Lei Wu, You-Hui Huang, Zhi-Quan Liu, Ping Yu, Pei-Hong Gu, Bin Fan, Yun-Long Zhao,