Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8318918 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2014 | 9 Pages |
Abstract
The immune system of cephalopods remains poorly understood. The aim of this study was to determine the specific activity of immune enzymes in epithelial barriers, circulatory and digestive systems of the common cuttlefish Sepia officinalis. Three enzyme groups with putative functions in immunity were investigated: phenoloxidases (POs), lysozymes and protease inhibitors (PIs). Consistent with a role in immunity, highest PO activities were found in the integument as well as the respiratory and circulatory organs under zymogenic (proPO) and active form. Surprisingly, high PO activities were also found in the digestive gland and its appendages. Similarly, high lysozyme activities were detected in the integument and circulatory organs, but also in the posterior salivary glands, highlighting the implication of this antibacterial enzyme group in most tissues exposed to the environment but also within the circulatory system. Albeit highest in digestive organs, the ubiquitous detection of PI activity in assayed compartments suggests immune function(s) in a wide range of tissues. Our study reports proPO/PO, lysozyme and PI distributions in S. officinalis body compartments for the first time, and thus provides the fundamental basis for a better understanding of the humoral immune system in cephalopods as well as invertebrates.
Keywords
Sepia officinalisBHABAPNADGASTCHcy3,4-dihydroxy-l-phenylalanineBSADMSOl-DOPAbovin serum albuminapostandard errorImmune systemDimethyl sulfoxideHEWProphenoloxidase systemDigestive glandphenoloxidaseLysozymesHen egg whiteCopperStomachTrypsin inhibitionProtease inhibitorsProtease inhibitorproPOHemocyaninPSGprophenoloxidase
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Charles Le Pabic, Georges Safi, Antoine Serpentini, Jean-Marc Lebel, Jean-Paul Robin, Noussithé Koueta,