Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8319105 | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | 2015 | 13 Pages |
Abstract
The influx of calcium ions (Ca2Â +) is responsible for various physiological events including neurotransmitter release and synaptic modulation. The L-type voltage dependent calcium channels (L-type VDCCs) transport Ca2Â + across the membrane. Calcium-binding proteins (CaBPs) bind free cytosolic Ca2Â + and prevent excitotoxicity caused by sudden increase in cytoplasmic Ca2Â +. The present study was aimed to understand the regulation of expression of neuronal CaBPs, namely, calretinin (CR) and parvalbumin (PV) following blockade of L-type VDCCs in the CNS of Carassius auratus. Verapamil (VRP), a potent L-type VDCC blocker, selectively blocks Ca2Â + entry at the plasma membrane level. VRP present in the aquatic environment at a very low residual concentration has shown ecotoxicological effects on aquatic animals. Following acute exposure for 96Â h, median lethal concentration (LC50) for VRP was found to be 1.22Â mg/L for goldfish. At various doses of VRP, the behavioral alterations were observed in the form of respiratory difficulty and loss of body balance confirming the cardiovascular toxicity caused by VRP at higher doses. In addition to affecting the cardiovascular system, VRP also showed effects on the nervous system in the form of altered expression of PV. When compared with controls, the pattern of CR expression did not show any variations, while PV expression showed significant alterations in few neuronal populations such as the pretectal nucleus, inferior lobes, and the rostral corpus cerebellum. Our result suggests possible regulatory effect of calcium channel blockers on the expression of PV.
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Authors
Nikhil V. Palande, Rahul C. Bhoyar, Saikat P. Biswas, Arun G. Jadhao,