Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8319497 | Current Opinion in Structural Biology | 2018 | 8 Pages |
Abstract
Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-throughput screening methods for rapid analysis of millions of variants are therefore increasingly important for investigating sequence-function relationships, searching large metagenomic libraries for interesting activities, and accelerating enzyme evolution in the laboratory. Recent applications of such technologies are reviewed here, with a particular focus on the practical benefits of droplet-based microfluidics for the directed evolution of natural and artificial enzymes. Broader implementation of such rapid, cost-effective screening technologies is likely to redefine the way enzymes are studied and engineered for academic and industrial purposes.
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Authors
Hans Adrian Bunzel, Xavier Garrabou, Moritz Pott, Donald Hilvert,