Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8319640 | Current Opinion in Structural Biology | 2016 | 10 Pages |
Abstract
In recent years, protein engineers have succeeded in tuning the excitation spectra of natural fluorescent proteins from green wavelengths into orange and red wavelengths, resulting in the creation of a series of fluorescent proteins with emission in the far-red portions of the optical spectrum. These results have arisen from the synergistic combination of structural knowledge of fluorescent proteins, chemical intuition, and high-throughput screening methods. Here we review structural features found in autocatalytic far-red fluorescent proteins, and discuss how they add to our understanding of the biophysical mechanisms of wavelength tuning in biological chromophores.
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Authors
Ho-Leung Ng, Michael Z Lin,