| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8319881 | Current Opinion in Structural Biology | 2015 | 8 Pages |
Abstract
Single-molecule Förster resonance energy transfer (smFRET) serves as a molecular ruler that is ideally posed to study static and dynamic heterogeneity in living cells. Observing smFRET in cells requires appropriately integrated labeling, internalization and imaging strategies, and significant progress has been made towards that goal. Pioneering studies have demonstrated smFRET detection in both prokaryotic and eukaryotic systems, using both wide-field and confocal microscopies, and have started to answer exciting biological questions. We anticipate that future technical developments will open the door to smFRET for the study of structure, conformational changes and kinetics of biomolecules in living cells.
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Authors
Marko Sustarsic, Achillefs N Kapanidis,