Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8319903 | Current Opinion in Structural Biology | 2015 | 10 Pages |
Abstract
- Single particle cryo-EM maps should be validated by specific tests.
- Tilt-pair analysis shows that a map is consistent with image data at low resolution.
- Map resolution should be consistent with map features and tests for over-fitting.
- Maps at low or high resolution may be validated by fitting an atomic model.
- Refinement of atomic models requires cross-validation tests to prevent over-fitting.
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Authors
Peter B Rosenthal, John L Rubinstein,