Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8322058 | The International Journal of Biochemistry & Cell Biology | 2018 | 12 Pages |
Abstract
The aim of the present study is to evaluate the effect of gamma linolenic acid (GLA) on mitochondrial mediated death apoptosis, hypoxic microenvironment and cholinergic anti-inflammatory pathway against 7, 12-dimethylbenz (a) anthracene (DMBA) induced mammary gland carcinoma. The effects of GLA were evaluated morphologically and biochemically against DMBA induced mammary gland carcinoma. The metabolic study was done for evaluation of biomarkers using 1H NMR. The present study was also verified through immunoblotting and qRT-PCR studies for the evaluation of various pathways. GLA treatment has a delineate implementation upon morphology of the tissues when evaluated through carmine staining, hematoxyline and eosin staining and scanning electron microscopy. GLA also demarked a commendatory proclamation of the fifteen key serum metabolites analogous with amino acid metabolism and fatty acid metabolism when recognized through1H NMR studies. The immunoblotting and qRT-PCR studies accomplished that GLA mediated mitochondrial death apoptosis, curtail hypoxic microenvironment along with hindrance of de novo fatty acid synthesis and also mediate the cholinergic anti-inflammatory pathway to proclaim its anticancer effects.
Keywords
AAAGSHECDCECTBARSDMBAGLAMPTPHIF-1αFADDNAGEBSSHBSSOAGHMDBTMDOPLS-DATRADDDcTLCTPHD2BMRBFas associated death domainCPMGMECDTTBSACarr–Purcell–Meiboom–GillHRVPCAbovine serum albuminBranched chain amino acidArachidonic acidAromatic amino acidfatty acid synthasePolyunsaturated fatty acidPUFAGamma linolenic acidelectrocardiographECGBCAAextracellular domainintracellular domaintransmembrane domainICDdithiothreitolSODMyoepithelial cellSuperoxide dismutaseHeart ratehypoxia inducible factor-1αtransmembranehigh frequencylow frequencyFasnlow density lipoproteinLDLHank’s balanced salt solutionSEMthiobarbituric acid reactive substancesScanning electron microscopyheart rate variabilityNitric oxideRIPhuman metabolome databaseReceptor interacting proteinprotein carbonylGlutathione
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Authors
Subhadeep Roy, Manjari Singh, Atul Rawat, Uma Devi, Swetlana Gautam, Rajnish Kumar Yadav, Jitendra Kumar Rawat, Md. Nazam Ansari, Abdulaziz S. Saeedan, Dinesh Kumar, Gaurav Kaithwas,