Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8322141 | The International Journal of Biochemistry & Cell Biology | 2017 | 13 Pages |
Abstract
Endocytosis, the formation of membrane vesicles from the plasma membrane, is an essential feature of eukaryotic cell biology. Intense research effort has been dedicated to developing methods that can detect endocytosis events with the highest resolution. We have classified these methods into four families. They exploit the physical properties of endocytosis, namely: 1. Distinguishing extracellular from internalised cargo in fixed samples, 2. Monitoring endosomal acidification, 3. Measuring the turnover of endocytic zones and 4. Detecting vesicle scission. The last three families, all based on fluorescence imaging, are used to study endocytosis in living cells. We discuss the advantages and limitations of these methods and conclude on the future developments required to tackle the upcoming challenges in this fundamental field of cell biology.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Morgane Rosendale, David Perrais,