Acute exposure of L6 myotubes to cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid isomers stimulates glucose uptake by modulating Ca2+/calmodulin-dependent protein kinase II

► CLA isomers stimulate (Cai2+) release. ► CLA isomer-mediated signals require both autoregulatory and calcium/calmodulin binding domains of CaMKII. ► Genetic knockdown of CaMKII prevents CLA-mediated glucose uptake. ► t10,c12-CLA stimulates skeletal muscle glucose transport via Cai2+-CaMKII-AMPK-AS160 ► Acute CLA-mediated ROS production does not modulate SAPK/JNK phosphorylation.