| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8326789 | International Journal of Biological Macromolecules | 2018 | 28 Pages |
Abstract
Releasing all monosaccharides during acid hydrolysis for composition analysis of polysaccharides has been a time consuming process. In current study, an efficient (10â¯Î¼L sampleâ¯+â¯10â¯Î¼L acid), sensitive, and quick monosaccharide composition analysis of polysaccharides was accomplished by using microwave-assisted HCl hydrolysis (10â¯min) of the polysaccharides followed by high-performance anion-exchange chromatography (HPAEC) combined with pulsed amperometric detection (PAD) analysis. Compared to the conventional hydrolysis procedure, this method is an efficient approach for monosaccharide composition analysis of acidic, basic, and neutral polysaccharides and particularly suited to polysaccharides that are difficult to hydrolyse fully such as chitosan, heparin and chondroitin sulfates.
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Authors
Yanli He, Meng Zhang, Ming Shan, Pengjiao Zeng, Xiulian Li, Cui Hao, Huaiqian Dou, Dandan Yang, Ningchuan Feng, Lijuan Zhang,