Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8326807 | International Journal of Biological Macromolecules | 2018 | 35 Pages |
Abstract
The proteome composition of Russell's viper venom (RVV) from southern India (SI) was investigated by 1D-SDS-PAGE of venom followed by tandem mass spectrometry analysis of protein bands. A total of 66 proteins belonging to 14 snake venom protein families were identified by LC-MS/MS analysis against Viperidae (taxid 8689) protein entries from the non-redundant NCBI database. Phospholipase A2 (43.25%) and snaclec (14.57%) represented the most abundant enzymatic and non-enzymatic proteins, respectively. SI RVV was characterized as containing a higher quantity of PLA2 and a lower amount of Kunitz-type serine protease inhibitors, in comparison to RVV from other regions of the Indian subcontinent. The enzymatic activities, pharmacological properties, and clinical manifestations of RV envenomation in SI were well correlated with its proteome composition; however, ATPase, ADPase, and hyaluronidase enzymes were not identified by LC-MS/MS analysis, owing to paucity of the existing database. Neurological symptoms exhibited by RV-bite patients in SI were correlated to the presence of abundant neurotoxic phospholipase A2 enzymes (15.66%) in SI RVV. Neutralization studies, immunological cross-reactivity, and antivenomics studies unequivocally demonstrated the poor recognition and lowest neutralization of PLA2 enzymes by commercial polyvalent antivenom, which is a major concern for the treatment of RV-envenomed patients in SI.
Keywords
SVSPPLBViperidaeCarboxypeptidaseARFPLA2ESI-LC-MS/MSSVMPNGFPaVADPPDEPro-coagulantBAEEAMPAPTTPolyvalent antivenom5′-NucleotidaseAntivenomicsl-amino acid oxidaseAdenosine TriphosphateATPadenosine diphosphateadenosine monophosphateRussell's viperphospholipase A2TameCRISPSouthern Indiaactivated partial thromboplastin timeprothrombin timeSnake venomSri LankaNeurotoxicVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)nerve growth factorPhosphodiesterasePhospholipase BLAAOAcute renal failureEastern IndiaWestern IndiaCysteine-rich secretory proteinglutaminyl cyclase
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Bhargab Kalita, Sudeepa Singh, Aparup Patra, Ashis K. Mukherjee,