Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327046 | International Journal of Biological Macromolecules | 2018 | 43 Pages |
Abstract
Long carbon chain alkanediols are used in the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], however these substrates possess high toxicity towards bacterial cells. This study demonstrated the effective utilisation of a long carbon chain alkanediol, namely 1,8-octanediol, to enhance the yield and production of a copolymer with a high molecular weight of over 1000â¯kDa, which is desirable for novel applications in medical and biopharmaceuticals. The increased PHA content (47-61â¯wt%) and concentration (1.7-4.5â¯g/L) was achieved by additional feeding of a combination of C4 substrates at C/N 10, with 1,8-octanediolâ¯+â¯Î³-butyrolactone producing P(3HB-co-22â¯mol% 4HB) with a high molecular weight (1060â¯kDa) and elongation at break of 970%. The DO-stat feeding strategy of C/N 10 has shown an increment of PHA concentration for both carbon combination, 0.45-4.27â¯g/L and 0.32-3.36â¯g/L for 1,8-octanediolâ¯+â¯sodium 4-hydroxybutyrate (4HB-Na) and 1,8-octanediolâ¯+â¯Î³-butyrolactone, but with a slight reduction on molecular weight and mechanical strength. Nonetheless, further study revealed that a nitrogen-absence feeding strategy could retain the high molecular weight and elongation at break of the copolymer, and simultaneously improving the overall P(3HB-co-4HB) production.
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Authors
Kai-Hee Huong, Elina K.A.R., Amirul A.A.,