Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327116 | International Journal of Biological Macromolecules | 2018 | 28 Pages |
Abstract
Monascus purpureus is known to produce several coloured secondary metabolites. In this study, M. purpureus CFR 410-11 mutant fermented with rice was dried and extracted in hexane for purification of pigment. The purity of the isolated pigment was confirmed by different chromatography techniques. The spectroscopic analysis revealed its structural identity as rubropunctatin. The antioxidant potencies of isolated rubropunctatin were evaluated. Rubropunctatin scavenged 16% 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and inhibited 20% superoxide generation at 8â¯Î¼g/ml concentration. The multiple antioxidant abilities of rubropunctatin were evidenced by its ferric reducing capacity also. The oxidative damage of BSA protein was induced by the metal catalyzed oxidation (MCO) by Fe2+/H2O2. The protective effects of rubropunctatin and M. purpureus (MTCC-410 and CFR 410-11) extracts were compared with glutathione and ascorbic acid. The M. purpureus extracts and rubropunctatin inhibited the formation of carbonyl content and protein oxidation assayed by SDS-PAGE. Rubropunctatin (42-169â¯Î¼M) efficiently inhibited the protein oxidation compared to glutathione (48-195â¯Î¼M) and ascorbic acid (85-340â¯Î¼M) by scavenging the superoxide and hydroxyl radical generated in the system.
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Biochemistry
Authors
Mohan A. Dhale, Manjunatha Javagal, Mohan-Kumari H. Puttananjaiah,