Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327153 | International Journal of Biological Macromolecules | 2018 | 11 Pages |
Abstract
In this present study, a gene (ent-B) encoding the bacteriocin enterocin-B was cloned, overexpressed and purified from Enterococcus faecium por1. The molecular weight of the bacteriocin enterocin-B was observed around 7.2â¯kDa and exhibited antimicrobial activity against several human pathogenic bacteria. The antimicrobial activity of cloned enterocin-B was increased effectively by combining with another bacteriocin enterocin-A from the same microorganism. Protein-protein docking and molecular dynamics simulation studies revealed that the bacteriocin enterocin-B is interacting with enterocin-A and formation of a heterodimer (enterocin Aâ¯+â¯B). The heterodimer of bacteriocin enterocin-Aâ¯+â¯B exhibited potential anti-bacterial, anti-biofilm activity against Staphylococcus aureus, Acinetobacter baumannii, Listeria monocytogenes and Escherichia coli. The bacteriocin enterocin-B, A and heterodimer of bacteriocin enterocin Aâ¯+â¯B showed no haemolysis on human RBC cells. This is the first report that the cell growth inhibitory activity of the bacteriocin enterocin B against HeLa, HT-29 and AGS human cancer cells and this cell growth inhibitory activity was significantly increased when cancer cells treated with the heterodimer of bacteriocins enterocin-Aâ¯+â¯B. The cell growth inhibitory activity of the bacteriocin enterocin-B and the heterodimer of bacteriocin enterocin-Aâ¯+â¯B were not observed in non-cancerous INT-407 cells (intestinal epithelial cells).
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Authors
Dasari Ankaiah, Esakkiraj Palanichamy, Christian Bharathi Antonyraj, Repally Ayyanna, Venkatesh Perumal, Syed Ibrahim Basheer Ahamed, Venkatesan Arul,