Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327418 | International Journal of Biological Macromolecules | 2018 | 35 Pages |
Abstract
This work was motivated by the need of stimuli responsive drug carriers, which can be activated by low cost non-invasive stimuli such as external magnetic field (EMF). Thus, novel antimicrobial materials based on xanthan gum (XG), magnetic nanoparticles (MNP), bovine serum albumin (BSA) and amoxicillin (Amox) were designed in order to promote the release of Amox under magnetic stimuli. Firstly, surfaces with different functionalities were prepared by sequential deposition of thin layers on Si wafers and characterized by means of ellipsometry and atomic force microscopy. Amox adsorbed preferentially onto XG or BSA films. In solution, favorable interactions between Amox and BSA were evidenced by substantial changes in the BSA secondary structure, as revealed by circular dichroism. Patches of XG and XG/MNP/BSA were immersed in 2â¯gâ¯Lâ1 Amox, yielding 10â¯Â±â¯3 and 17â¯Â±â¯4â¯Î¼g/cm3 Amox loading, respectively. The inclusion of 0.2â¯wt% Fe3O4 in the patches and their exposure to EMF enabled in vitro release of Amox, at pHâ¯5.5 and 0.02â¯molâ¯Lâ1 NaCl, following the quasi-Fickian behavior. Amox diffused from XG/MNP/BSA patches in agar medium containing Staphylococcus aureus and Escherichia coli, inhibiting their growth. The inhibition of E. coli growth was particularly efficient under EMF.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Pedro V.A. Bueno, Karina C.P. Hilamatu, Ana M. Carmona-Ribeiro, Denise F.S. Petri,