Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327534 | International Journal of Biological Macromolecules | 2018 | 6 Pages |
Abstract
Peroxynitrite (ONOOâ) is a reactive oxidant involved in numerous pathological conditions. Thymoquinone (TQ) is an active constituent of Nigella sativa and is reported to have anti-disease activities, but its role on ONOOâ has never been investigated. This study was undertaken to investigate the role of TQ on ONOOâ-induced damage of histone-H2A. Our novel data showed TQ significantly inhibited ONOOâ-induced oxidative damage in histone-H2A. ONOOâ induces UV-hypochromicity of histone-H2A, whereas TQ reversed this effect to hyperchromicity. Tyrosine fluorescence was significantly reduced by ONOOâ and was significantly increased upon TQ treatment. TQ reduces ONOOâ-induced hydrophobicity in histone-H2A and also reduces thermal stability of ONOOâ-histone H2A complex. SDS-PAGE of native histone-H2A showed a single band, which disappeared when treated with ONOOâ alone. This changed was retained when protein samples were treated with TQ. Similar protective effects of TQ were found when protein carbonyl contents were estimated. In conclusion, this is the first study that shows the potential of TQ against ONOOâ-induced damaged of histone-H2A. TQ inhibits oxidative modification of tyrosine, lysine, arginine, proline and threonine in histone-H2A. These results have importance for the development of novel therapeutic strategies for the treatment of disorders, where ONOOâ plays a role.
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Authors
Zafar Rasheed, Abdullah A. Altorbag, Abdulilah S. Al-Bossier, Nasser A. Alnasser, Omar S. Alkharraz, Khalid M. Altuwayjiri, Abdulaziz S. Alobaid, Ahmad K. Alsaif, Yazeed H. Alanazi, Bassam A. Alghidani, Muath A. Alduayji, Ali A. Bu Mozah,