Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327748 | International Journal of Biological Macromolecules | 2018 | 8 Pages |
Abstract
In our present work, the polysaccharides (MPS) were successfully extracted from the mycelia of Hypsizygus marmoreus, and purpose of our work was to evaluate the analysis of the characterization, anti-inflammation and anti-oxidation against the lung failure induced by lipopolysaccharide (LPS) in mice. Pretreatment with MPS at 800 mg/kg markedly ameliorated the lung wet-to-dry weight (W/D) ratio and pulmonary histopathological conditions, reduced the tumor necrosis factor-alpha (TNF)-α, interleukin (IL)-6, and IL-1β levels in bronchoalveolar lavage fluid (BALF), myeloperoxidase (MPO) activities. In addition, the protective effects of MPS might be attributed to the down-regulations of the serum complement 3 (C3), glutamyl transpeptidase (GGT) and high-sensitivity C-reactive protein (hs-CPR) contents, as well as improving the antioxidant status by enhancing pulmonary enzyme activities (catalase (CAT), superoxide dismutase(SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC)) and eliminating the lipid peroxidation (LPO) and malondialdehyde (MDA), respectively. What's more, our work investigated the characterization of the MPS.
Keywords
CATIL-1βHDL-CVLDL-CPDAhs-CRPHypsizygus marmoreusIL-6LPSGGTLDL-CT-AOCLPOMDAnuclear magnetic resonanceMPODMSOGSH-PxH&EROSW/Dstandard deviationinterleukin-6Interleukin-1βBALFtriglycerideNMRCharacterizationtumor necrosis factor-alphaSODSuperoxide dismutasepotato dextrose agarFT-IRTotal antioxidant capacityTNF-αhigh-density lipoprotein cholesterollipopolysaccharidemalondialdehydeBronchoalveolar lavage fluidSEMcomplement 3myeloperoxidaseScanning electron microscopyMPsweight-average molecular weightLipid peroxidationhigh-sensitivity C-reactive proteinCatalaseHPLChigh performance thin layer chromatographyGas chromatographytotal cholesterolVery low-density lipoprotein cholesterollow density lipoprotein cholesterolNormal controlPositive controlModel controlHydroxyl groupsglutathione peroxidaseglutamyl transpeptidaseReactive oxygen species
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Authors
Min Liu, Shangshang Li, Xiuxiu Wang, Yongfa Zhu, Jianjun Zhang, Hui Liu, Le Jia,