Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8327886 | International Journal of Biological Macromolecules | 2018 | 10 Pages |
Abstract
Intercellular Candida glabrata infections are difficult to treat due to poor penetration of drugs into the fungal niche. Delivering amphotericin B (Amp B) into the macrophages where the pathogen inhabits is an effective solution. We are studying the macrophage targeting proficiency of É©-carrageenan for the delivery of Amp B using gelatin A nanoparticles (GNPs). The choice of gelatin A was the outcome of in silico inspections where the amino functionalized polymer having the best docking score with Amp B was selected. We prepared a sustained release formulation of amp B loaded carboxymethyl É©-carrageenan conjugated gelatin nanoparticles (CMC-Amp B-GNPs) with size 343 ± 12 nm and â25 ± 5.3 mV zeta potential. The formulations were found to be stable, biocompatible and non-haemolytic. Flow cytometry analysis showed 3 fold higher uptake of CMC-GNPs compared to the GNPs by RAW 264.7 cells. CMC-Amp B-GNPs showed enhanced antifungal activity than bare Amp B and Amp B-GNPs.
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Authors
V. Aparna, Anu Rohit Melge, V.K. Rajan, Raja Biswas, R. Jayakumar, C. Gopi Mohan,