Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8328454 | International Journal of Biological Macromolecules | 2018 | 27 Pages |
Abstract
Rheumatoid factor (RF) is an auto-antibody against antigen-antibody immune complexes. RF is valuable as a biomarker for the screening of autoimmune and infectious diseases. However, it is suggested that RF would be a more powerful biomarker when used complementarily with RF-correlated proteins. In this study, we utilized a proteomic approach to analyze global protein expression in RF-low and RF-high subjects using high-performance liquid chromatography tandem mass spectrometry. Histidine-rich glycoprotein (HRG) and lipopolysaccharide-binding protein (LBP) were found to be differentially expressed between RF-low and RF-high subjects (cut-off >â¯2-fold, pâ¯<â¯0.05), which was validated by enzyme-linked immunosorbent assay. To evaluate whether both proteins allow discriminating rheumatoid arthritis patients from healthy controls, receiver-operating characteristic (ROC) curves were analyzed. Areas under the ROC curves of HRG and LBP were 0.861 and 0.888, respectively. The correlation between RF and HRG was statistically significant (pâ¯=â¯0.003), and LBP was also correlated with RF (pâ¯=â¯0.044), as indicated by correlation analysis. HRG and LBP are reportedly involved in RF-producing and RF-correlated diseases. Thus, we propose that HRG and LBP could be useful screening markers for RF-correlated diseases.
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Authors
Doojin Kim, Sora Mun, Jiyeong Lee, Arum Park, AeEun Seok, Yeon-Tae Chun, Hee-Gyoo Kang,