Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8328600 | International Journal of Biological Macromolecules | 2018 | 9 Pages |
Abstract
Roots of Sophora flavescens is an important herbal medicine for treatment of HBV and hepatic carcinoma in China. Alkaloids in the root were well known for exhibiting good hepato-protective and anti-HBV effects. However, polysaccharides as main components in the root remained unknown. In the studies, we investigated the chemical features and hepatoprotective effects of Sophora flavescens polysaccharides (SFP-100 and its active fractions) with ConA-induced hepatitis mice, human liver LO2 cells and HepG2.2.15 cells. The results showed that SFP-100 was composed of arabinose, glucose, galactose and galacturonic acid, SFP-100-A mainly contained glucose. SFP-100-B and SFP-100-C were acidic polysaccharides. SFP-100 significantly decreased hepatocytes apoptosis, inhibited the infiltration of neutrophils and macrophages into liver, and improved the production of IFN-γ and IL-6 of splenocytes in ConA-induced hepatitis mice. SFP-100 and its two sugar fractions increased LO2 cell proliferation and reduced cell apoptosis induced by ConA. SFP-100, SFP-100-A and SFP-100-C remarkedly inhibited the secretion of HBsAg and HBeAg by HepG2.2.15 cells.These results suggested Sophora flavescens polysaccharides exerts significant hepatoprotective and anti-HBV roles, and further is used for treatment of immune-mediated liver disease in the future.
Keywords
HPGPCTFASophora flavescensArabinoseGlcIFN-γDCsConcanavalin AIL-12FBSJnkDMEMConAc-Jun N-terminal kinaseDMSODulbecco’s modified Eagle mediumMTTTrifluoroacetic acidGalacturonic acidCapillary electrophoresisinterferon-gammainterleukin-12Interleukin-1βEnzyme-linked immunosorbent assayELISAtumor necrosis factor αARAfetal bovine serumDendritic cellsVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)TNF-αhematoxylin–eosinHBVhepatitis B virusPolysaccharidesGalGalAGalactoseGlucose
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Authors
Hua Yang, Zhenhua Zhou, Lifang He, Hao Ma, Wensheng Qu, Jiye Yin, Mengfan Jia, Xiunan Zhao, Junjie Shan, Yueqiu Gao,