| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8328637 | International Journal of Biological Macromolecules | 2018 | 7 Pages |
Abstract
Cancer antigen 15-3 (CA15-3) is a key biomarker, currently used for understanding the onset and prognosis of breast cancer. In present investigation, CA15-3 has been purified from the culture supernatant of breast cancer T47-D cell line with 76% yield and 3350 fold purification. Isolated CA15-3 was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting (western blotting), chemiluminescence immunoassay (CLIA) and Fourier-transform infrared spectroscopy (FTIR). CA15-3 is a monomeric protein with an apparent molecular mass in between â¼250-350Â kDa. The FTIR spectroscopy revealed similar profiles of T47-D derived CA15-3 and commercially available CA15-3 protein. With the easy availability of T47-D cell line and a simple purification approach described here will support for the large scale production of CA15-3 to be used for various clinical and diagnostic applications.
Keywords
HRPCLIAMUC1ICMRTMBATCCPAGEHETPkiloDaltonkDaPBSTMWCOTCAPESCancer antigen 15-3PBS with 0.05% Tween 20FBSSDSpower of hydrogenDMEMIL-6PBSPC-1Polyethersulphone3,3′,5,5′-tetramethylbenzidineBSAIU/mlDulbecco's modified Eagle's mediumTffbovine serum albuminalpha fetoproteinCancer antigenHeight equivalent to a theoretical platetrichloroacetic acidpolyacrylamide gel electrophoresisimmunoglobulin chemiluminescence immunoassayinterleukin-6AFPEnzyme-linked immunosorbent assayELISATangential flow filtrationHourBreast cancerfetal bovine serumIndian Council of Medical ResearchFTIRPhosphate buffered salineFourier-transform infraredAmerican Type Culture CollectionMucin 1Molecular Weight Cut OffHorseradish peroxidase
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Shoaib Haidar, Paresh B. Bhanushali, Kunal K. Shukla, Deepak Modi, Chander P. Puri, Shamkant B. Badgujar, Manoj Chugh,