Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8329205 | International Journal of Biological Macromolecules | 2017 | 26 Pages |
Abstract
The blue-green microalga, Arthrospira sp., isolated from the sea of Kssour Essef in Mahdia (Tunisia), was purified and then identified both morphologically and genetically based on 16S rRNA gene sequence. Following physicochemical analysis, the prokaryotic microalga tested represented a competitive source of pigments and showed a considerable rate in protein (64%) which was confirmed by FTIR measurement. The lipid content (4%) was quantified by the gravimetric method and the intracellular lipid bodies were detected with the Nile red staining. Using gas chromatography coupled with flame ionization detector, the fatty acid profile revealed the presence of 27.4% and 32.88% of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs), respectively. Given the richness of the isolated microalga in unsaturated fatty acids, we have developed a SYBR Green real time PCR method for the specific identification of Arthrospira sp. Î9 desaturase gene. This current method will be of great value for carrying out high-throughput studies like cloning, heterologous expression and structure-function relationship analysis.
Keywords
PBSArthrospira sp.RFUPUFAsDEPCRT-PCRTLCDPPHNCBIBHT2,2-diphenyl-1-picrylhydrazylDNAenzyme linked immunosorbent assayBasic Local Alignment Search Tooldesoxyribonucleic acidpoly-unsaturated fatty acidsUltravioletBlastFourier transform infraredTriacylglycerolsTAG یا triacylglycerols ELISAflame ionization detection16S rDNA sequencingFIDdiethylpyrocarbonateFTIRPhosphate buffered salineFatty acid methyl esterFAME یا fatty acid methyl esters National Center for Biotechnology InformationNile Redbutylated hydroxytolueneRelative Fluorescence Unitsdry weightLipidsthreshold cyclethin layer chromatographyGas chromatography
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Authors
Faten Ben Amor, Mohamed Barkallah, Fatma Elleuch, Nesrine Karkouch, Mouna Dammak, Bruno Baréa, Pierre Villeneuve, Slim Abdelkafi, Imen Fendri,