Article ID Journal Published Year Pages File Type
8329210 International Journal of Biological Macromolecules 2018 18 Pages PDF
Abstract
Recent studies on crude and pure compounds from Sargassum horneri have shown promising bioactive properties. However, anti-inflammatory potentials of fucose-rich sulfated polysaccharides from S. horneri have not yet been discovered. In the present study, we evaluated the in vitro and in vivo anti-inflammatory activities of four polysaccharide fractions separated from membrane filters according to their molecular weights (<5 kDa (f1), 5-10 kDa (f2), 10-30 kDa (f3), and >30 kDa (f4)). According to the results, F4 fraction inhibited the lipopolysaccharides (LPS)-induced nitric oxide (NO) (IC50 = 87.12 μg/mL) and prostaglandin E2 production as well as pro-inflammatory cytokine production in RAW 264.7 cells through down-regulating nuclear factor-κB signaling cascade. According to the results, f4 has a potential to down-regulate LPS-induced toxicity, cell death and NO production levels in LPS-induced in vivo zebrafish embryo model. These results suggest that f4 fraction has the potential to develop functional materials or drugs to treat inflammatory diseases.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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