Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8329412 | International Journal of Biological Macromolecules | 2017 | 32 Pages |
Abstract
In the present study, the chitosan-enriched magnetic composites (MCCs) were prepared by a novel and simple embedding method for the immobilization of α-glucosidase (α-Glu). The immobilized α-Glu could be easily separated from the reaction mixture under an external magnetic field owing to the magnetic support. With the MCCs-immobilized α-Glu, enzyme activity and stability were studied, and enzyme inhibitors were screened from traditional Chinese medicines (TCMs) and vegetables combined with capillary electrophoresis (CE). The MCCs-immobilized α-Glu exhibited enhanced pH and temperature tolerance with unchanged optimum pH and temperature of 4.0 and 60 °C comparing with free α-Glu. Reusability of the immobilized α-Glu was significantly improved after immobilization, and it retained 62.2% of its initial activity after 10 repeated cycles. Immobilized α-Glu also showed improved storage stability (84.3 ± 1.2% after 35 days of storage at 4 °C). The kinetic parameter Km for immobilized α-Glu was calculated to be 0.81 mM and the affinity of enzyme towards its substrate was reduced after immobilization. Finally, immobilized α-Glu was used to screen enzyme inhibitors from the extracts of TCMs and vegetables. The enhanced pH and temperature tolerance, improved reusability and storage stability of MCCs-immobilized α-Glu make it a promising candidate for biotechnological applications.
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Authors
Dong-Mei Liu, Juan Chen, Yan-Ping Shi,