Lipolytic bacteria use as bio-decontaminating natural in the water purification stations

Different samples were collected from polluted waters by fat materials in the Mascara region for the isolation of bacterial strains capable of degrading fat materials and used in the production of lipase by the fermentation method. A total of two strains (Pseudemonas sp. et Streptococcus sp.) were isolated at 37 °C from the sample water rich slaughterhouses fat materials. Production of extracellular bacterial lipase was studied as a function of several inductors of lipid nature by the fermentation method. The enzyme activity reached a maximum value, in the presence of olive oil as inducer. The enzyme was purified by precipitation with ammonium sulfate in a yield of 63.73% and 50% in Streptocoques sp. and Pseudemonas sp. respectively. The lipase produced by these two bacteria is resistant to 50 °C and is strongly inhibited in the presence of 1 mmol Zn+2 and Mg +2.