Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8330118 | International Journal of Biological Macromolecules | 2015 | 37 Pages |
Abstract
Heat shock protein 90 (Hsp90) is a highly conserved ubiquitous molecular chaperone contributing to assisting folding, maintenance of structural integrity and proper regulation of a subset of cytosolic proteins. In the present study, a heat shock protein 90α full length coding cDNA was isolated and cloned from the Arabian one-humped camel by reverse transcription polymerase chain reaction (RT-PCR). The full length cDNA sequence was submitted to NCBI GeneBank under the accession number KF612338. The sequence analysis of the Arabian camel Hsp90α cDNA showed 2202 bp encoding a protein of 733 amino acids with estimated molecular mass of 84.827 kDa and theoretical isoelectric point (pI) of 5.31. Blast search analysis revealed that the C. dromedarius Hsp90α shared high similarity with other known Hsp90α. Comparative analyses of camel Hsp90α protein sequence with other mammalian Hsp90s showed high identity (85-94%). Heterologous expression of camel Hsp90α cDNA in E. coli JM109 (DE3) gave a fusion protein band of 86.0 kDa after induction with IPTG for 4 h.
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Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Hesham Saeed, Manal Shalaby, Amira Embaby, Mohammad Ismael, Akbar Pathan, Farid Ataya, Mohammad Alanazi, Khalid Bassiouny,