Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8330357 | International Journal of Biological Macromolecules | 2015 | 29 Pages |
Abstract
Hydrolysis of xyloglucan from Tamarindus indica and Hymenaea courbaril seeds with endoglucanase (EGII), which randomly breaks the (1 â 4)-linked β-glycosidic bonds of the polymer chain, was monitored in real time using time-dependent viscometry analysis (TDV). For both samples there was a decrease in the intrinsic viscosity ([η]), viscosity average molar mass (Mv), radius de gyration (Rg) and persistence length (Lp) immediately after the addition of the enzyme. It was observed the formation of oligosaccharides and oligomers composed of â¼2 units, up to 140 min. Galactose-containing side chains two positions away from the non-substituted glucose, modulated the action of EGII, and the complete hydrolysis of the XG oligomers occurred after 24 h. The results demonstrate for the first time the real-time degradation of xyloglucan as well the macromolecular and oligosaccharide composition during the EGII hydrolysis process.
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Authors
Vivian Cristina Spier, Maria Rita Sierakowski, Amid Tony Ibrahim, Jéssica C. Scholze Baum, Joana Lea M. Silveira, Rilton Alves de Freitas,