Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8330372 | International Journal of Biological Macromolecules | 2015 | 10 Pages |
Abstract
An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a cross-linking agent. The effects of cross-linking were analyzed by Fourier transform infrared spectroscopy. The results indicate that the homogeneous materials blending and cross-linking intensity were dependent on the molecular weights of COSs. The highly interconnected porous architecture with 160-260 μm pore size and over 90% porosity and COS's MW driven swelling and retention capacity, tensile property and in vitro biodegradation behavior guaranteed the FCA/COS scaffolds for skin tissue engineering application. Further improvement of these properties enhanced the cytocompatibility of all the scaffolds, especially the scaffolds containing COSs with MW in the range of 1-3 kDa (FCA/COS1) showed the best cytocompatibility. These physicochemical, mechanical, and biological properties suggest that the FCA/COS1 scaffold is a superior candidate that can be used for skin tissue regeneration.
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Authors
Pathum Chandika, Seok-Chun Ko, Gun-Woo Oh, Seong-Yeong Heo, Van-Tinh Nguyen, You-Jin Jeon, Bonggi Lee, Chul Ho Jang, GeunHyung Kim, Won Sun Park, Wonseok Chang, Il-Whan Choi, Won-Kyo Jung,